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Oligonucleotide primers and probes, for the detection of hepatitis B virus, are used to amplify, by polymerase chain reaction, a section of the hepatitis B virus genome
Oligonucleotide primers and probes, for the detection of hepatitis B virus, are used to amplify, by polymerase chain reaction, a section of the hepatitis B virus genome
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机译:用于检测乙肝病毒的寡核苷酸引物和探针用于通过聚合酶链反应扩增乙肝病毒基因组的一部分
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摘要
A method of detecting hepatitis B virus (HBV) in a sample comprising exposing the sample to a polymerase chain reaction (PCR), where the PCR amplified DNA fragment corresponds to part of the HBV genome in the region of positions 242-482 of the HBV genome, counted from the single EcoRI cleavage site in the genome, is new. Independent claims are also included for the following: (1) oligonucleotides chosen from (I)-(IV), and complements of (III) and (IV); (2) a plasmid containing a DNA fragment having a 382 bp sequence, fully defined in the specification; and (3) a test kit to detect HBV containing an oligonucleotide composition, the necessary nucleotides for PCR and a heat stable DNA polymerase. CnAACCTSYGTCCTCCAAYTTGT (I); GATGAGGCATAGCAGCAGGAT (II); AACGCCGCAGACACATCCAGCGAT (III); and ACGACGGACCACACTGACCAGTCA (IV). n = 0 or 1; S = C or G; and Y = C or T.
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机译:一种检测样品中的乙型肝炎病毒(HBV)的方法,包括将样品暴露于聚合酶链反应(PCR)中,其中PCR扩增的DNA片段对应于HBV基因组242-482区域中的部分HBV基因组从基因组中单个EcoRI切割位点算起的基因组是新的。还包括以下方面的独立权利要求:(1)选自(I)-(IV)的寡核苷酸,以及(III)和(IV)的互补物; (2)一种质粒,其含有说明书中完全定义的具有382bp序列的DNA片段。 (3)一种检测试剂盒,用于检测含有寡核苷酸组合物,PCR所需的核苷酸和热稳定的DNA聚合酶的HBV。 CnAACCTSYGTCCTCCAAYTTGT(I); GATGAGGCATAGCAGCAGGAT(II); AACGCCGCAGACACATCCAGCGAT(III);和ACGACGGACCACACTGACCAGTCA(IV)。 n = 0或1; S = C或G; Y = C或T。
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