New heparichinaze, the manufacturing method and its production germ

摘要

PURPOSE:To obtain the subject new enzymes useful as a low-molecular heparin- forming agent, etc., in preparing a low-molecular heparin such as an antithrombotic agent by culturing a bacterium, belonging to the genus Bacillus and having the ability to produce heparitinases T-I to T-IV and collecting the resultant product from a culture solution or a microbial cell extract solution. CONSTITUTION:A bacterium, belonging to the genus Bacillus and having the ability to produce heparitinase T-I, heparitinase T-II, heparitinase T-III and heparitinase T-IV is cultured and the resultant product is then collected from its culture solution or a microbial extract solution. Thereby, the objective new enzymes heparitinase T-I (optimum pH; 5.5-6.5, optimum temperature; 55 deg.C), heparitinase T-II (optimum pH; 5.5-6.5, optimum temperature; 55 deg.C), heparitinase T-III (optimum pH; 7.0-8.0, optimum temperature; 50 deg.C) and heparitinase T-IV (optimum pH; 7.0-8.0, optimum temperature; 40 deg.C) which are lyases, acting on glycosaminide bonds of heparin and heparan sulfate and forming double bonds between the 4- and 5-positions of glucuronic acid or iduronic acid at the cleaved site are obtained.