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CULTURE MEDIUM COMPOSITION FOR CULTURING PHELLINUS LINTEUS MYCELIUM AND CULTURE OF PHELLINUS LINTEUS MYCELIUM USING THE SAME COMPOSITION
CULTURE MEDIUM COMPOSITION FOR CULTURING PHELLINUS LINTEUS MYCELIUM AND CULTURE OF PHELLINUS LINTEUS MYCELIUM USING THE SAME COMPOSITION
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机译:用于培养桑白球菌的培养培养基组合物和使用该组合物培养桑白球菌的培养基
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摘要
PROBLEM TO BE SOLVED: To provide a culture medium composition for culturing Phellinus linteus mycelium in which the culture of Phellinus linteus mycelium is easily conducted and a special process for extracting mycelium is needless, and to provide a method for producing Phellinus linteus mycelium using the same culture medium. SOLUTION: This method comprises the following steps: preparing a culture medium by formulating 40-60 wt.% of at least one kind selected from the group consisting of brown rice, roasted brown rice, barley (or wheat), its powder, and uncracked and roasted grains of barley with 40-60 wt.% of underground water, by pouring the mixture into a bottle for culturing spawn, by heating them at a steam pressure of 15 lb/cm2 (120-121 deg.C) for 50-60 min to perform high pressure sterilization and by cooling them; separating tissue from carpophore of natural Phellinus linteus, subculturing the tissue in a medium extracted from mulberry trees and then performing seed culture in a similar medium; and inoculating the seed culture solution in the above formulated culture medium, followed by culturing it at 25±10 deg.C for 25-30 days.
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