Rotaviruses non-structural protein 4 (nsp4) antigenic composition, processes to protect a mammalian host against disease caused by rotaviruses, and to improve diarrheal symptoms in a mammalian host, isolated and purified nucleic acid sequence, plasmid containing a nucleic acid sequence isolated and purified host cell and process to produce an alternative form of rotavirus nsp4

摘要

'' NON-STRUCTURAL PROTEIN 4 (NSP4) FROM ROTAVÍRUS ANTIGENIC COMPOSITION, PROCESSES TO PROTECT A MAMMALIAN GUEST AGAINST DISEASE CAUSED BY ROTAVIRUS, AND TO IMPROVE HUMOROUS, HUMOROUS, HUMOROUS WOMEN IN A HOSPITAL ISOLATED AND PURIFIED NUCLEIC ACID, HOST CELL, AND PROCESS TO PRODUCE AN ALTERNATIVE FORM OF ROTAVIRUS NSP4 ''. The non-structural protein 4 (NSP4) in the ATCC SA11 rotavirus strain has a histidine at the amino acid position 47. This substituted form is more cytotoxic than the NSP4 of the Australia rotavirus strain, which has an asparagine at the amino acid position 47. A histidine at amino acid position 47 is mutagenized to another amino acid to produce an alternative form of NSP4 that has reduced toxicity, while maintaining its antigenicity and immunogenicity. Because NSP4 has a glutamic acid at amino acid position 48 it is more cytotoxic than NSP4 with a lysine at amino acid position 48. Lysine at amino acid position 48 is mutagenized to another amino acid other than glutamic acid to produce an alternative form of NSP4 that has reduced toxicity, while maintaining its antigenicity and immunogenicity.