Method for immobilizing a polysaccharide on a solid surface, solid surface, use of it, test device for detecting antibodies against one or more gram-negative bacteria, method for estimating the number of bacteria or for determining serotype of bacterial antigen, compound, use and method for preparing it

摘要

"METHOD FOR IMMOBILIZATION OF A POLYSACARIDE IN A SOLID SURFACE, SOLID SURFACE, USE OF THE SAME, TEST DEVICE FOR THE DETECTION OF ANTIBODIES AGAINST ONE OR MORE GRAM-NEGATIVE BACTERIA OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATUS OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF THE STATEMENT OF BACTERIAL ANTIGEN, COMPOUND, USE AND METHOD FOR THE PREPARATION OF THE SAME ". The present invention provides a method for immobilizing a polysaccharide (PS) on a solid surface, said polysaccharide having a keto-carboxy group ((-C (= O) = COOH) or a corresponding ketal or hemicetal group) , for example derived from KDO (2-keto-3-deoxy-D-manooctonic acid), the method comprising the steps of: (a) forming a covalent bond between the polysaccharide carboxy group and a reporter molecule (RM), comprising a recognition / substrate site (for example biotin or an anthroquinoma); and (b) immobilize for diagnostic purposes, for example for the detection of bacterial infections of gram-negative bacteria.