PRODUCTION OF FUCOSYLATED CARBOHYDRATES BY ENZYMATIC FUCOSYLATION SYNTHESIS OF SUGAR NUCLEOTIDES; AND IN SITU REGENERATION OF GDP-FUCOSE

摘要

This invention-contemplates improved methods of enzymatic production ofcarbohydrates especially fucosylatedcarbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using bothchemical and enzymatic means are also contemplated. Thephosphorylated glycosides are then used to produce sugar nucleotides that arein turn used as donor sugars for glycosylation ofacceptor carbohydrates. Especially preferred herein in the use of a disclosedmethod for fucosylation.