VEGF FUSION PROTEIN AND EXPRESSION VECTOR THEROF FOR TREATMENT OF VEGF DEPENDENT DISEASE

摘要

PURPOSE: A VEGF(vascular endothelial growth factor) fusion protein and expression vector thereof for treating VEGF dependent disease are provided. The vector expresses VEGF in high yield when transfected into eukaryotes, especially mammary culture cells and when injected into the tissue in vivo. CONSTITUTION: An expression vector for eukaryotes, which expresses fusion protein, is characterized by jointing partial gene or whole gene which codes a fusion protein, into partial gene or whole gene which codes VEGF isoform protein. The gene coding fusion protein is characterized by immunoglobulin gene(IgG). The IgG gene is characterized by Fc section of human IgG. The VEGF gene is cDNA of VEGF121 protein and expression vector pCN-VEGF/Fc for eucaryotes is represented by the figure 2. Transformed recombinant E.coli strain, Top10-pCN-VEGF/Fc (KFCC-11082) is transformed with the pCN-VEGF/Fc vector.