MONOCLONAL ANTIBODY SPECIFIC FOR HUMAN UPSTREAM REGULATORY ELEMENT BINDING PROTEIN 1 AND FUSION CELL STRAIN PRODUCING SAME

摘要

PURPOSE: Provided is a monoclonal antibody specifically binding to upstream regulatory element binding protein 1(UREB1) which is specifically expressed in cancer cell and tissue. And a fused cell line producing the same is also provided. Therefore, liver cancer can be easily diagnosed by using them. CONSTITUTION: The monoclonal antibody and the fused cell line producing the same are manufactured by the steps of: manufacturing an expression vector, plasmid pET28a-UREB1, to produce recombinant protein of UREB1(Histag-UREB1) by transforming E.coli BL21(lambda DE3) therewith; separating and purifying UREB1 by subjecting the culture of transformants to centrifugation, ultrasonification add electrophoresis sequentially; immunizing a mouse by injecting a mixture of UREB1 and Freund complete adjuvant(FCA) into a mouse and 2 weeks after, injecting a mixture of UREB1 and Freund incomplete adjuvant(FIA) again; fusing a mouse spleen cell and SP2/0Ag14 cell line as a parent cell to obtain fused cell line; and selecting a fused cell line which produces a monoclonal antibody by ELISA using UREB1 as an antigen, wherein two fused cell lines, KY0603 (KCTC 0689BP) and KY0800 (KCTC 0690BP) are screened.