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62 62 8957 213/ 8956 62 Discovery of Staphylococcus haemolyticus L62KCTC 8957P producing a novel lipase and development of its efficient production method using Escherichia coli BL21DE3/pSHMLKCTC 8956P
62 62 8957 213/ 8956 62 Discovery of Staphylococcus haemolyticus L62KCTC 8957P producing a novel lipase and development of its efficient production method using Escherichia coli BL21DE3/pSHMLKCTC 8956P
The present invention is activated by a non-ionic surfactant to be produced from the by psychrotrophic lipase excellent bow performance novel strain Staphylococcus H. Mori T carcass (Staphylococcus haemolyticus) L62 (KCTC 8957P ) and such a novel strain isolated from soil greatly increases and L62 lipase and then transformed E. coli BL21 to mass produce the above-mentioned L62 lipase using the T7 promoter (DE3) / pSHML (KCTC 8956P) and resource L62 lipase using (resource) Q column having a high activity even at low temperatures the present invention relates to purification methods.
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机译:本发明由非离子型表面活性剂激活,该非离子表面活性剂是由嗜营养型脂肪酶的弓性能优异的新型菌株葡萄球菌H.Mori T. car体(Staphylococcus haemolyticus)L62(KCTC 8957P I>)制得的。从土壤中分离的菌株大大增加,L62脂肪酶然后转化大肠杆菌BL21,使用T7启动子(DE3)/ pSHML(KCTC 8956P)大量生产上述L62脂肪酶,使用(资源)Q柱的资源L62脂肪酶具有即使在低温下也具有高活性的方法本发明涉及纯化方法。
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