INFLUENZA VIRUS STRAIN A/47/SYDNEY/97/14 (H3N2) FOR PRODUCTION OF LIVE INFLUENZAL INTRANASAL VACCINE FOR BABIES

摘要

FIELD: applied microbiology. SUBSTANCE: vaccinal strain A/47/Sydney/97/14 (H3N2), which is reassortant, is obtained by cross-breeding of epidemic virus A/Sydney/5/97/ (H3N2) with cold-adapted temperature-sensitive virus A/Leningrad/134/47/57 (H2N2), which is attenuation donor. Strain A/47/Sydney/97/14 (H3N2) actively multiplies in developing chicken embryos at optimal temperature 34 C. Strain is characterized by temperature sensitivity and cold adaptability. Reassortant inherits two genes from epidemic virus, which encode surface proteins (gemmagglutinin and neuraminidase). It also receives six genes encoding non-glycosylated proteins from attenuation donor. In addition, the strain does non induce immune response in babies during intranasal injection. Biological properties and low degree of immunoreactivity meet requirements for vaccine strains. EFFECT: enabled prophylactics of epidemic influenza provoked by specific antigenic virus species. 1 tbl