METHOD FOR THREE-STRANDED DNA FORMATION, THREE- STRANDED DNA AND SOUTHERN HYBRIDIZATION

摘要

PROBLEM TO BE SOLVED: To provide a method for forming a three-stranded DNA stable even in the absence of protein, etc., to obtain the three-stranded DNA and to provide a southern hybridization using the three-stranded DNA. SOLUTION: This method for three-stranded DNA formation has a process for forming a DNA-protein complex in which a complementary domain of single-stranded DNA is bonded to an end neighborhood inclusion domain of double-stranded DNA in a participation state of at least a homological recombination protein, etc., by reacting a straight-chain double-stranded DNA with a straight-chain single-stranded DNA containing a base sequence substantially complementary to a part of the double-stranded DNA, a homological recombination protein, etc., and exonuclease I, etc., and a process for forming a three- stranded DNA having a three strand formation domain in which a complementary domain of single-stranded DNA is bonded to an end neighborhood inclusion domain of double-stranded DNA by deactivating the protein.