Measurement method of anti-neutrophil cytoplasmic antibodies

摘要

PURPOSE: To measure a chromatic antineutrophilic cytoplasmic antibody (C- ANCA) accurately by retarding the nonspecific reaction due to myeloperoxidase (MPO) antigen coexisting with the antibody. ;CONSTITUTION: When the IgG fraction of an animal derived anti-human MPO antiserum is added to a C-ANCA antigen, it has no effect on the measurement of C-ANCA but only the measurement of MPO-ANCA lowers because the IgG fraction of anti-human neutrophilic MPO animal antiserum reacts previously on the MPO antigen to retard reaction between the MPO-ANCA and the MPO antigen. Since the nonspecific reaction of MPO antigen is retarded, the MPO- ANCA is prevented from being mismeasured as C-ANCA due to the MPO antigen coexisting with the C-ANCA antigen resulting in the accurate measurement of C-ANCA.;COPYRIGHT: (C)1995,JPO