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MANGANESE PEROXIDASE SOLUTION, MANGANESE PEROXIDASE, AND METHOD FOR PRODUCING MANGANESE PEROXIDASE

机译:锰过氧化物酶溶液,锰过氧化物酶和生产锰过氧化物酶的方法

摘要

PROBLEM TO BE SOLVED: To provide a manganese peroxidase having especially excellent heat resistance and excellent hydrogen peroxide resistance.;SOLUTION: This manganese peroxidase which is obtained by culturing SC21 strain that is a variant prepared by irradiating Phanerochaete crysosporium with ultraviolet light, and has a residual activity of ≥50% after heated at 50°C for 45 min and also a residual activity of ≥50% after treated in the presence of 0.3 mM hydrogen peroxide solution at 37°C for one hr. Three kinds of isozymes constituting the concrete contents of the manganese peroxidase. The manganese peroxidase solution containing the isozymes. The method for producing the manganese peroxidase, comprising collecting the enzyme solution or the enzyme from the culture product of the above-described SC21 strain.;COPYRIGHT: (C)2002,JPO
机译:解决的问题:提供具有特别优异的耐热性和优异的过氧化氢抗性的锰过氧化物酶;解决方案:该锰过氧化物酶是通过培养SC21菌株而获得的,该菌株是用紫外线照射Phanerochaete低温孢子菌制备的变体,并且具有在50℃下加热45分钟后,残余活性为约50%,在0.3mM过氧化氢溶液中于37℃下处理1小时后,残余活性为约50%。三种同工酶构成了锰过氧化物酶的具体成分。含过氧化物的锰过氧化物酶溶液。生产锰过氧化物酶的方法,其包括从上述SC21菌株的培养产物中收集酶溶液或酶。版权所有:(C)2002,JPO

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