BREEDING OF STRAIN HIGHLY PRODUCING ISOAMYL ACETATE, AND APPLICATION THEREOF

摘要

PROBLEM TO BE SOLVED: To provide a method for breeding a strain by which a high-quality alcohol drink containing a large amount of isoamyl acetate, and having excellent fragrance can be produced. SOLUTION: A mutated yeast formed by mutation, having high alcohol acetyl transferase activities, and high ratio of isoamyl acetate/isoamyl alcohol, capable of producing a large amount of the isoamyl acetate is bred and isolated. The novel mutated yeast can be obtained from the strain grown in a selective medium containing a steroid having a pregnane skeleton having a hydroxylated 3-position such as pregnenolone, 17-hydroxypregnenolone and pregnanediol, or a sterol such as cholesterol.