Recombinant host cell, methods for enzymatically degrading an oligosaccharide, to identify a substitute promoter capable of increasing the expression of a gene of interest in a host cell, to produce a recombinant host cell for use in simultaneous saccharification and fermentation, to produce a integral recombinant host cell, to express a polysaccharase in a host cell and to produce ethanol from an oligosaccharide source, and, vector

摘要

"RECOMBINANT HOSPEDEIRA CELL, METHODS FOR ENZYMATICALLY DEGRADING AN OLIGOSACARID, TO IDENTIFY A SUBSTITUTE PROMOTER ABLE TO INCREASE THE EXPRESSION OF A GENE-OF-INTEREST IN A HOSPITAL CELL, TO PRODUCE A HERITAGE AND TO PRODUCE A CULTURE AN INTEGRATING RECOMBINANT HOSTING CELL, TO EXPRESS A POLYSACCHASE IN A HOSTING CELL AND TO PRODUCE ETHANOL FROM AN OLIGOSACARID SOURCE, AND, VECTOR ". The invention provides recombinant host cells, containing at least one heterologous polynucleotide encoding a polysaccharase under the transcriptional control of a substitute promoter, capable of increasing polysaccharase expression. In addition, the invention further provides such hosts with genes encoding secretory protein (s), to facilitate secretion of the expressed polysaccharase. The preferred hosts of the invention are ethanological and capable of carrying out simultaneous saccharification and fermentation, resulting in the production of ethanol from complex cellulose substrates.