Processes to increase the yield of a recombinant protein produced in insect cell culture and recombinant pestiviruses and fragments or proteins therein and to produce recombinant follicle stimulating hormone, alpha-units and / or beta-units, complexes and fragments of the same use of a process, and, vaccine.

摘要

"PROCESSES TO INCREASE THE YIELD OF A RECOMBINANT APAPROTEIN PRODUCED IN A CULTURE OF RECOMBINANT INSECT AND PESTIVIRUS CULTURE E2O FRAGMENTS OR PROTEIN E ^ RNS ^ OF THE SAME AND TO PRODUCE HOMENEOUS STIMULATING, FOMINES AND HOMINOMINISTS, HOMINOMINES AND HOMINOMINISTS USE OF A PROCESS, AND, VACCINE ". The invention relates to a process for increasing protein expression in bacillus vector virus expression systems. The invention provides a process for producing a recombinant protein in insect-cell culture that comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel with a volume sufficient to contain at least 2 liters and infecting cells with inoculum of at least one baculovirus at a cell density of 1 x 10 ^ 5 ^ to 10 ^ 6 ^ cells / ml with one mol 0.01. The invention furthermore provides a process for producing recombinant pestiviruses of E2 or E ^ rns ^ protein or same fragment in insect cell culture, characterized by a final concentration of said protein (fragments) in the medium of crop growth at least 100 109 g / ml. The invention furthermore provides a process for producing recombinant follicle stimulating hormone, 244 -units and / or 225 -units and complexes and fragments thereof, at a concentration in the growth medium of at least 15 109 / ml harvest.