EFFICIENT PRODUCTION OF MULTIPLE SHOOTS FROM THIDIAZURON-TREATED MATURE EMBRYOS AND LEAF-BASE/APICAL MERISTEMS OF BARLEY (HORDEUM VULGARE L.) GENOTYPES

摘要

The in vitro plant regeneration frequencies for immature scutella, leaf-bases/apicalmeristems (LB/AM) and mature embryos of four commercially important barleygenotypes were compared. Production of shoots from mature embryos or calli ofLB/AM incubated on media containing 1.0 or 2.0 mg/L 6-benzyiaminopurine (BAP)were comparable to regeneration frequencies obtained for scutella-derivedcalli of thesame genotypes. Incubation of excised mature embryos and LB/AM on mediacontaining an alternative plant growth regulator, thidiazuron (TDZ), resultedin anincreased shoot production. However, TDZ treatment did not stimulate plantregeneration from calli derived from scutella or LB/AM. Shoots formed from TDZ-treated mature embryos and LB/AM were induced without a callus interphase andthein vitro culture system gave a three- to sight-fold higher regenerationfrequency thanrecorded for scutella-derived calli on BAP medium. The simplicity and rapiddevelopment of shoots using the mature embryo system strongly favours its useovercommonly used regeneration systems.