Procedure for the long-term cultivation of organo-typic slices of brain and other postnatal tissues, in particular of adult mammals

摘要

Method for long-term culture of organotypic slices (A) of brain or other tissues from postnatal, especially adult, mammals, is new. (A) is deposited on a porous membrane, and culture medium applied to a flat surface or to a well in a device designed to accommodate the membrane. The membrane, loaded with (A), is then applied to the medium, and suction applied from (A) to the medium. Method for long-term culture of organotypic slices (A) of brain or other tissues from postnatal, especially adult, mammals, is new. (A) is deposited on a porous membrane, and culture medium applied to a flat surface or to a well in a device designed to accommodate the membrane. The membrane, loaded with (A), is then applied to the medium, and suction applied from (A) to the medium. Alternatively, the membrane, with (A), is applied to the flat surface or the device, then the medium introduced underneath the membrane.