Method for screening of anti-cervical cancer agents and apoptotic modulating factors

摘要

The present invention for cervical cancer and apoptosis regulators search method, E. coli BL21 / pET28a-E7 (E.coli, BL21 / pET28a-E7) who expressed the GST and His- converged at KCTC 18016P papillomavirus (human Papillomavirus; HPV) 16 type E7 to rapidly purify recombinant proteins with glutathione beads and nickel beads, to a person soluble fraction of the cancer suppressor Rb and reaction production in E. coli the binding capacity by Western blotting method and Ella and (ELISA) method by checking the service E7-Rb in vitro binding capacity and the search method, there is excellent effect that can be used in the search and development and screening of anticancer and apoptosis regulator through the search method.