Recombinant microorganisms expressing a fusion protein of Escherichia coli enterotoxin II signal peptide and fusion protein of human growth hormone and a method of producing human growth hormone using the same

摘要

The present invention provides a recombinant microorganism expressing a fusion protein of E. coli enterotoxin II signal peptide that promotes the secretion of foreign protein expressed in E. coli cells into the surrounding cytoplasm, and a human growth hormone using the same. It is about a method. According to the present invention, by expressing a variant of E. coli enterotoxin II signal peptide and human growth hormone in the form of a fusion protein improved by the genetic engineering technology to have a higher secretion efficiency, without using a separate protein expression derivative Human growth hormone of the type can be obtained with high efficiency. Therefore, by expressing the signal peptide of the present invention in the form of various useful proteins and fusion proteins, including human growth hormone, it will be possible to produce a natural useful protein having the same function as that expressed in vivo in high yield.