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RECOMBINANT PLASMID DNA PHSVD1 DETERMINING EXPRESSION OF GENE US6 FRAGMENT OF HERPES SIMPLEX TYPE-1 VIRUS ENCODING IMMUNO-DOMINANT MOIETY OF GLYCOPROTEIN D(GD)HSV-1 IN BACTERIUM ESCHERICHIA COLI CELLS
RECOMBINANT PLASMID DNA PHSVD1 DETERMINING EXPRESSION OF GENE US6 FRAGMENT OF HERPES SIMPLEX TYPE-1 VIRUS ENCODING IMMUNO-DOMINANT MOIETY OF GLYCOPROTEIN D(GD)HSV-1 IN BACTERIUM ESCHERICHIA COLI CELLS
FIELD: biotechnology, genetic engineering, molecular biology. SUBSTANCE: invention relates to recombinant plasmid DNA constructed in vitro and containing gene US6 fragment of herpes simplex type-1 virus (HSV-1) that encodes immunodominant moiety of glycoprotein D (gD) of HSV-1 virus from 269 to 392 amino acid residue. Construction provides biosynthesis in bacterium E. coli cells the polypeptide as a fragment of β--galactosidase of E. coli (from 1 to 376 amino acid residue) with polyhistidine (6-His) tract for affinity purification. This polypeptide shows antigenic properties of herpes simplex virus of type-1. Purified recombinant protein can be used as HSV-1 antigen for serological testing HSV-1 in clinical practice. EFFECT: recombinant DNA and protein indicated above, valuable properties. 5 dwg, 3 ex
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机译：领域：生物技术，基因工程，分子生物学。发明内容本发明涉及体外构建的重组质粒DNA，其包含单纯疱疹1型病毒（HSV-1）的基因US6片段，该基因编码HSV-1病毒糖蛋白D（gD）从269至392个氨基酸残基的免疫优势部分。构建提供了在细菌大肠杆菌细胞中的生物合成，该多肽是大肠杆菌的β-半乳糖苷酶的片段（1至376个氨基酸残基），具有多组氨酸（6-His）束，用于亲和纯化。该多肽显示1型单纯疱疹病毒的抗原特性。纯化的重组蛋白可用作HSV-1抗原，在临床实践中用于血清学检测HSV-1。效果：上述重组DNA和蛋白质，有价值的特性。 5 dwg，3 ex
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