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Detecting differences between nucleic acid populations, useful e.g. for differential expression studies, based on competitive hybridization with excess of probes
Detecting differences between nucleic acid populations, useful e.g. for differential expression studies, based on competitive hybridization with excess of probes
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机译:检测核酸群体之间的差异,例如用于差异表达研究,基于与过量探针的竞争性杂交
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摘要
Process for detecting differences between two populations (T, test; E, screen) of single-stranded nucleic acids. The complements of T are synthesized and divided into two equal fractions, I and II and: (a) I is hybridized to a set of nucleic acid probes; and (b) II is combined with E, before, or concomitantly with, hybridization to the set of probes. The hybridization results for (a) and (b) are compared.
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