ANTI-5-METHYL-2'-DEOXYCYTIDINE ANTIBODY AND METHOD FOR MEASURING 5-METHYL-2'-DEOXYCYTIDINE

摘要

PROBLEM TO BE SOLVED: To provide a highly specific antibody against 5-methyl-2'-deoxycytidine, to provide a method for effectively producing a hybridoma producing the antibody, and to provide a specific and highly sensitive direct competitive enzyme immunization method against a methylated DNA containing 5-methyl-2'- deoxycytidine.;SOLUTION: This method for producing a hybridoma producing a highly specific antibody against the 5-methyl-2'-deoxycytidine is characterized by fusing a myeloma cell with a spleen cell by an electrical fusion method. The hybridoma produced by the method. And the direct competitive enzyme immunization method against the methylated DNA is characterized by using a highly specific antibody against the 5-methyl-2'-deoxycytidine or its fragment.;COPYRIGHT: (C)2003,JPO