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Method for influencing pollen development by modifying sucrose metabolism

机译:通过修饰蔗糖代谢影响花粉发育的方法

摘要

Methods for influencing pollen development by modifying the sucrose metabolism in transgenic plant cells and plants and generating male sterile plants by sucrose depletion in pollen. An expression of a protein has enzymatic activity of a sucrose isomerase in transgenic plant cells. Nucleic acid molecules contain a DNA sequence encoding a protein having the enzymatic activity of a sucrose isomerase, wherein the DNA sequence is functionally linked with the regulatory sequences of a promoter active in plants so that the DNA sequence is expressed in anthers or pollen. The invention further relates to transgenic plants and plant cells that contain the inventive nucleic acid molecule and whose male plant and plant cells are sterile due to the expression of the DNA sequence that encodes a protein having the enzymatic activity of a sucrose isomerase. The invention also relates to harvest products and the propagation material of said transgenic plants.
机译:通过改变转基因植物细胞和植物中的蔗糖代谢并通过花粉中蔗糖消耗产生雄性不育植物来影响花粉发育的方法。蛋白的表达在转基因植物细胞中具有蔗糖异构酶的酶促活性。核酸分子包含编码具有蔗糖异构酶的酶促活性的蛋白质的DNA序列,其中该DNA序列与植物中有活性的启动子的调节序列功能性连接,从而该DNA序列在花药或花粉中表达。本发明进一步涉及含有本发明核酸分子的转基因植物和植物细胞,其雄性植物和植物细胞由于编码具有蔗糖异构酶酶活性的蛋白质的DNA序列的表达而是无菌的。本发明还涉及所述转基因植物的收获产品和繁殖材料。

著录项

  • 公开/公告号US2003159181A1

    专利类型

  • 公开/公告日2003-08-21

    原文格式PDF

  • 申请/专利权人 BORNKE FREDERIK;SONNEWALD UWE;

    申请/专利号US20020223277

  • 发明设计人 UWE SONNEWALD;FREDERIK BORNKE;

    申请日2002-08-14

  • 分类号A01H1/00;C07H21/04;C12N9/24;C12N15/82;C12N5/04;

  • 国家 US

  • 入库时间 2022-08-22 00:10:03

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