Method for studying protein-protein interactions

摘要

The present invention provides reagents, kits and methods for identifying and characterizing interactions between proteins and/or polypeptides. The inventive system and methods allow analysis of these interactions in vivo in eukaryotic systems, including mammalian systems. Advantages provided by various embodiments of the inventive system and methods as compared with other systems and methods for analyzing interactions between proteins or polypeptides, such as the yeast two-hybrid system, include (i) reduced ambiguity associated with identification of a potential interaction; (ii) ability to identify or study interactions that occur outside the nucleus; (iii) absence of reliance on reporter genes; and/or (iv) ability to study interactions with polypeptides that have transcriptional activation activity.