Process for generating cytoplasmic male sterile line in rice and other crops by rna editing

摘要

The present invention relates to a cytoplasmic male sterility (CMS) transgenic plant prepared by RNA editing for expressing unedited nad 9 gene disenabling ATP production in the mitochondria of plants and a process for preparing the same. The said process comprises: cloning an un-edited nad 9 gene by digesting the PCR product obtained from mitochondrial DNA to obtain pNG3 cloning crop with un-edited nad 9 gene with the targeting sequence under ubiquitin promoter a NOS terminator to obtain pNG11 co-bombarding pNG11 constructs in a manner as herein described with hygromycin gene (pLAU6 hph construct) to generate plants containing un-edited nad 9 gene; analysing un-edited nad 9 (pNG11) construct with a control plant having edited nad 9 gene, pNG10 to determine the presence of nad 9 gene.