Compositions for freezing dog sperm and method of freezing the dog sperm utilizing the compositions and artificial insemination method employing the frozen dog sperm

摘要

Disclosed herein is a composition and method for freezing dog sperm. The composition is based upon 100 ml of distilled water, 0.25 to 4.5 g of sodium citrate, 0.45 to 4.0 g of dextrose, 0.01 to 0.3 g of penicillin, 50,000 to 500,000 IU of streptomycin. 0.25 to 0.45 g of catalase, 0.045 to 0.25 g of yolk, 1.5 to 15 ml of glycerol and 0.02 to 0.1 ug of dog serum. The method for freezing dog sperm includes the steps of (a) collecting male dog sperm by a massage method or electrical shock, (b) centrifuging the collected sperm to separate and remove the upper fluid leaving only the lower sperm, (c) mixing equal volumes of the lower sperm and the composition and primarily refrigerating the mixture, (d) further adding the composition to the mixture obtained in the step (c) to reduce the volumetric ratio of sperm to the composition to 1:2, and secondarily refrigerating the resulting mixture, and (e) adding liquid nitrogen to the mixture obtained in the step (d) and freezing the mixture.