Construct, method of generating transgenic plant or progeny resistant to ssdna virus, linear genetic element and genetically modified avian species and plant or part of it

摘要

"CONSTRUCTION, GENERAL METHOD OF GENERATING TRANSGENIC PLANT OR PROGENY RESISTANT TO ssDNA VIRUS, LINEAR GENETIC ELEMENT, AND GENETICALLY MODIFIED PLANT, OR PART THEREOF". The present invention relates generally to constructs and, in particular, to genetic constructs comprising polynucleotide sequences capable of releasing, in circular, covalently closed form, from a larger nucleotide sequence such as, but without limitation, a genome of a eukaryotic cell. Preferably, once released, a polynucleotide sequence is reconstituted into a form that allows expression of the polynucleotide sequence. In one embodiment, the reconstituted polynucleotide sequence comprises a coding sequence with all or part of a foreign nucleotide such as, but not limited to, an intronic sequence or other junction signal inserted therein. The expression and, in particular, the coding sequence transcription involves joining the strange sequence. Release and circularization is generally in response to a stimulus such as a protein mediated stimulus. More particularly, the protein is a prokaryotic or eukaryotic cell or virus derived protein or developmentally and / or tissue specific regulated protein. The construct of the present invention is particularly useful for conferring resistance against pathogens or apoptosis induction or other mechanisms of cell death useful, for example, in treating cancer or inducing male or female sterility in plants. The constructs therefore allow site specific expression and / or developmentally regulated expression of selected genetic sequences.