Nucleic acid sequence, nucleic acid, expression vector, host cell, methods for enhancing binary toxin production of b. sphaericus in a bacterial host cell, to create a recombinant bacterium, to increase the toxicity of a b. thuringiensis to a mosquito larva, recombinant cell of b. sphaericus, methods for increasing the toxicity of a b. sphaericus, and, to reduce resistance to a binary toxin of b. spharericus

摘要

"NUCLEIC ACID SEQUENCE, NUCLEIC ACID, EXPRESSION VECTOR, HOST CELL, METHODS FOR IMPROVING B. SPHAERICUS BINARY TOXIN PRODUCTION IN A BACTERIAL ARIARY BRITERIAL CABIN TO A CRITIC BIOTERIAL TO A CRITIC CARRIER THURINGIENSIS TO A MOSQUITO SUGAR, B. SPHAERICUS RECOMBINANT CELL, METHODS TO INCREASE TOXICITY OF A B. SPHAERICUS CELL, AND TO REDUCE RESISTANCE TO A BINARY TOXIN OF B. SPHAERICUS ". The invention relates to nucleic acid sequences comprising a BtI or BTII promoter, or a combination of BtI promoter and a BtII, at least 6 contiguous nucleotides of a bacterial STAB-SD sequence, and a sequence encoding a polypeptide having at least 80% sequence identity for a 41.9 kD protein of B. sphaericus binary toxin ("Bs"), whose polypeptide has at least 50% the toxicity of the native 41.9 kD protein. Optionally, the sequence further encodes a second polypeptide with at least 80% sequence identity for the 51.4 kD binary toxin protein Bs and which may function as a binding domain for the 41.9 toxin protein. When the nucleic acid sequences are expressed in B. thuringiensis ("Bt") cells, they result in at least 10 times the production of Bs binary toxin compared to untransformed Bs cells. The invention provides nucleic acid sequences, expression vectors, host cells, and methods for increasing the toxicity of an insecticidal bacterium by transforming the bacterium with nucleic acid sequences of the invention. Furthermore, the invention relates to the discovery that the Bt subspecies israelensis Cyt1Aa1 protein ("Bti") reverses the binary toxin resistance of Bs in larvae of BS resistant mosquitoes. The invention provides Bs cells expressing Bt Cyt1Aa1 protein. Bti and methods for reducing Bs binary toxin resistance by co-administering CytlAal protein with Bs binary toxin.