Novel furanic labdane diterpene compounds from Vitex rotundifolia L. fruit process for extraction and Acyl-CoA cholesterol acyltransferase inhibitors including thereof

摘要

PURPOSE: A composition containing rotundifuran and 6-epi-rotundifuran as a furanic labdane diterpene compound extracted from a fruit of Vitex rotundifolia Linne fil. is provided. Because the rotundifuran and 6-epi-rotundifuran specifically inhibits ACAT, it is effective in lowering blood cholesterol level and can be effectively used in prevention and treatment of cardiovascular disease caused by hypercholesterolemia. CONSTITUTION: ACAT(acyl-CoA:cholesterol acyltransferase) activity inhibitor contains a furanic labdane diterpene compound such as rotundifuran of formula(1) or 6-epi-rotundifuran of formula(2). The fruit of Vitex rotundifolia Linne fil. is soaked in methyl alcohol at room temperature for 3 days and concentrated under reduced pressure to produce a crude extract. The crude extract is fractioned with n-hexane, chloroform, ethyl acetate and water to concentrate a n-hexane layer and then separated by chromatography.