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- Vector for expression of mouse granulocyte-macrophage colony stimulating factor and a transformant transformed with the vector
- Vector for expression of mouse granulocyte-macrophage colony stimulating factor and a transformant transformed with the vector
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机译:-用于表达小鼠粒细胞-巨噬细胞集落刺激因子的载体和用该载体转化的转化体
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摘要
PURPOSE: A vector for the expression of mouse granulocyte-macrophage colony stimulating factor and a transformant transformed with the same are provided, thereby mass-producing the recombinant mouse granulocyte-macrophage colony stimulating factor. CONSTITUTION: A vector pGM-CSF over-expresses the mouse granulocyte-macrophage colony stimulating factor and has a cleavage map shown in figure 4. A transformant E. coli BL21 (DE3)/pGM-CSF(KCTC 10123BP) is produced by transformation with the vector pGM-CSF. The mouse granulocyte-macrophage colony stimulating factor is produced by culturing the transformant E. coli BL21 (DE3)/pGM-CSF(KCTC 10123BP), and recovering the mouse granulocyte-macrophage colony stimulating factor from the cultured medium.
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