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Method for Increasing Humanized Antibody Production in CHO Cells by Overexpression of bcl-2 Gene and Treatment of Sodium Butyrate
Method for Increasing Humanized Antibody Production in CHO Cells by Overexpression of bcl-2 Gene and Treatment of Sodium Butyrate
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机译:通过bcl-2基因的过表达和丁酸钠的处理增加CHO细胞中人源化抗体产生的方法
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摘要
PURPOSE: Provided is a method for increasing humanized antibody production in CHO cells by overexpression of bcl-2 gene and treatment of sodium butyrate, thereby increasing unit production of animal cell products, such as humanized antibodies and cytokines. CONSTITUTION: The antibody production is increased by introducing a vector which overexpresses bcl-2 gene into CHO cells transformed with a gene encoding a humanized antibody to obtain a cell line having apoptosis resistance, then adding sodium butyrate. The method for increasing the production of humanized antibodies comprises the steps of: introducing a vector which overexpressing human bcl-1 gene into the recombinant CHO cells above; inoculating the recombinant CHO cells transformed with the vector to a medium; and adding sodium butyrate thereto.
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