BALLISTIC METHOD OF SUNFLOWER (HELIANTHUS ANNUUS L) TRANSGENIC PLANT PREPARING

摘要

FIELD: plant selection, molecular biology, genetic engineering. SUBSTANCE: for preparing sunflower transgenic plants there are transformed with exogenous DNA inserted in vegetable cells on solid carrier particles. Then sprouts are regenerated from cells. Exogenous DNA can be presented, for example, genes with resistance to antibiotics or herbicides. The use of promoter RTL2 for gene expression determining resistance to hygromycin and selection of transformed of cells in liquid phase ensure to obtain high percent of sunflower transformants. EFFECT: improved method of transgenic plants preparing. 6 cl, 2 dwg, 1 tbl, 9 ex