New short streptavidin-binding peptides, for use as tags for purification of proteins, or as detection labels for labeling proteins

摘要

Streptavidin-binding proteins (I) containing, or consisting of, any of 12 specified amino acid sequences, are new. New streptavidin-binding proteins (I) contain, or consist of, any of 12 specified amino acid sequences of formulae S1 - S12. Independent claims are also included for the following: (1) nucleic acid (II) that encodes (I); (2) a plasmid that contains (II); and (3) producing (I) by (over)expression of (II) in a cellular or cell-free protein biosynthesis system. Asp-Val-Glu-Ala-Trp (S1) Asp-Val-Glu-Ala (S2) Val-Glu-Ala-Trp (S3) Asp-Val-Glu (S4) Val-Glu-Ala (S5) Glu-Ala-Trp (S6) Asp-Val-X1-Ala-Trp (S7) Asp-Val-X2-Ala-Trp-Leu (S8) Asp-Val-X3-Ala-Trp-Leu-X4 (S9) Asp-Val-X5-Ala-Trp-Leu-X6-X7 (S10) Asp-Val-X8-Ala-Trp-Leu-X9-X10-Arg (S11) Asp-Val-X11-Ala-Trp-Leu-X12-X13-Arg-Val-Pro-Leu-Val-Glu-Thr (S12) each X = any amino acid.