NEW THERMOSTABLE PROTEIN HAVING SLIDING CLAMP FUNCTION OF DNA POLYMERASE

摘要

PROBLEM TO BE SOLVED: To obtain new thermostable protein having sliding clamp function of DNA polymerase.;SOLUTION: The protein composed of a specific amino acid sequence has sliding clamp function of DNA polymerase. The protein is obtained by cloning a gene estimated to encode protein having sliding clamp function of DNA polymerase from a gene sequence of Sulfolobus tokodaii species 7(JCM10545) which is a hyperthermophilic archaebacterium and a kind of aerobic thermoacidophilic crenarchaeon and expressing the cloned gene using Escherichia coli. The figure 2 is the SDS-PAGE photograph of the protein.;COPYRIGHT: (C)2005,JPO&NCIPI