PROBLEM TO BE SOLVED: To enable a keratinized cell layer to be proliferated on the upper surface in cell culture, if necessary, a fibroblast layer to be cultured on the upper surface of the keratinized cell layer, to enable a cultured epithelium/epidermis and a cultured mucous membrane/skin having a target size to be produced without causing shrinkage during the culture period, to provide a tough cell culture substrate having flexibility, and to provide the manageable, flexible and tough cultured epithelium/epidermis and cultured mucous membrane/skin enabling the application and suture to be easily carried out well.;SOLUTION: The cultured mucous membrane/skin is produced by using a cell culture substrate 1 using a tough and unshrinkable sheet having the flexibility as a base and having a collagen coated film 1b formed on the upper surface of the base sheet 1a, seeding a keratinizing cell (a) on the upper surface of the collagen-coated film 1b, culturing the seeded keratinizing cell (a) to form the keratinized layer A, seeding a fibroblast cell b on the upper surface of the keratinized cell layer A, culturing the fibroblast cell to form the fibroblast cell layer B. The cultured mucous membrane/skin is applied by applying the membrane/skin to a defective part of the whole layer of the mucous membrane or the skin so that the fibroblast cell layer may be directed downward and the outer face may be brought into contact with the defective part at the transplanting.;COPYRIGHT: (C)2004,JPO
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