TAXONOMIC IDENTIFICATION OF PATHOGENIC MICROORGANISM AND TOXIC PROTEIN OF PATHOGENIC MICROORGANISM

摘要

PPROBLEM TO BE SOLVED: To provide a method for binding pathogenic microorganisms and their toxic toxins with ligands. PSOLUTION: The ligands are covalently tethered at some distance from the surface of a substrate. Distances at least 15 are required for microorganism binding ligand tethers and at least 6 are required for protein binding ligand tethers. The ligands include heme compounds, siderophores, polysaccharides, and peptides specific for toxic proteins, outer membrane proteins, and conjugated lipids. Non-binding components of the solution to be analyzed are separated from the bound fraction. Binding can be detected via microscopy, fluorescence, epifluorescence, luminescence, phosphorescence, radioactivity, or optical absorbance. By patterning numerous ligands in an array on a substrate surface, it is possible to taxonomically identify the microorganism by analysis of the binding pattern of the sample to the array. PCOPYRIGHT: (C)2005,JPO&NCIPI