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METHOD FOR INTRODUCING GENE INTO E. COLI OR THE LIKE, CAPABLE OF BEING USED AS SCIENCE EXPERIMENT IN COMPULSORY EDUCATION
METHOD FOR INTRODUCING GENE INTO E. COLI OR THE LIKE, CAPABLE OF BEING USED AS SCIENCE EXPERIMENT IN COMPULSORY EDUCATION
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机译:将基因导入大肠杆菌或类似物种的方法,可以用作义务教育中的科学实验
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摘要
PROBLEM TO BE SOLVED: To provide a very simple method for introducing a polynucleotide into a prokalyotic cell in a sufficient efficiency and capable of being used e.g. as a school education in an elementary or a middle school.;SOLUTION: This very simple method for introducing a gene which can be performed by anyone, is characterized by performing the gene introduction to the prokayotic cell having a cell wall consisting mainly of peptidoglycan in the presence of a transfection reagent such as cation liposome, etc., and a polynucleotide, and also introducing the polynucleotide into the cell by adding a complex of the transfection reagent with polynucleotide to the prokayotic cell cultured in an activating medium and then culturing the cell in a selection medium.;COPYRIGHT: (C)2004,JPO
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