The method of refining the rearrangement protein which is revealed as the insoluble cohesion body

摘要

This invention is revealed in the bacterial host cell, solubilizes the rearrangement protein which it accumulates as the insoluble cohesion body (enclosure body) regards the method in order to refine. Refinement is based on the transformation which uses chromatography method making use of, the organic denaturation reagent to dissolved form of the enclosure body. The inorganic alkaline salt content eluent, is selected here, but after because of this, refinement completes, it rearranges and the protein after the neutralizing, it is possible, to use directly for medical use, with the form which it can allow physiology it can make available. This method is suited, although especially, the allergen and the allergen fragment are refined.