Nucleic acids and methods for detecting viral infection, uncovering anti-viral drug candidates and determining drug resistance of viral isolates

摘要

A nucleic acid construct is provided. The nucleic acid construct includes an expression cassette including: a first polynucleotide region including a 5′ NCR sequence of an RNA virus and at least an N-terminal portion of a core sequence of the RNA virus; a second polynucleotide region including a 3′ UTR sequence of the RNA virus and at least a C-terminal portion of a polymerase sequence of the virus; and a third polynucleotide region encoding a reporter molecule, the third polynucleotide region being flanked by the first and the second polynucleotide regions; and a promoter sequence being operatively linked to the expression cassette in a manner so as to enable a transcription of a minus strand RNA molecule from the expression cassette.