Mutations of cryptic splice sites in cre and cre fused proteins for improvement of expression and inducibility

摘要

The invention relates to a DNA sequence, which codes for a mutant of the bacteriophage P1 recombinase, in which a particular cryptic splicing site is disabled by means of a base mutation which alters the Cre protein sequence. The invention further relates to a DNA sequence which codes for a fusion protein from the named Cre mutants and a ligand-binding domain of a receptor protein. The invention furthermore relates to vectors, micro-organisms and transgenic organisms, containing such a DNA sequence and Cre mutants or Cre fusion proteins which are coded by said DNA sequence.