An isolated DNA encodes a monomeric anthranilate synthase, vector, Transgenic Plant Seed and understands it; The Method for altering the content of tryptophan in the Plant; The Method for Human Food and animal feed or food animal or human obtained.

摘要

A method for altering the content of tryptophan in a Plant through the introduction and expression of an isolated segment of DNA that encodes a monomeric anthranilate synthase in the cells of the plant.The anthranilate synthase monomer monomer can be naturally, including and not limited to agencies such as Agrobacterium tumefaciens meliloti, Rhizobium, Mesorhizobium loti, Brucella melitensis, Nostoc sp. PCC 7120, Azospirillum brasilense and Anabaena m22983.Alternatively, the anthranilate synthase monomer can be a merger of any available domain of anthranilate synthase Alpha and beta. Such Domains Alpha and beta can be derived from Zea mays, soya, Rice, Cotton, Wheat, Tobacco, or any Gene that Codes for a subunit or domain of anthranilate synthase.The isolated DNA may also encode for anthranilate synthase Mutant or a domain of anthranilate synthase Mutant. Transgenic plants transformed with a DNA segment isolated that encode an anthranilate synthase, as well as food for humans or animals, Seeds and Progeny from these plants.Claim 1: an isolated DNA encoding an anthranilate synthase Monomer, wherein the anthranilate synthase monomer comprises a single polypeptide with a domain of anthranilate synthase Alpha and beta domain of anthranilate synthase, and where the anthranilate synthase mo Nomu00e9rica is expressed in a Plant.Claim no. 9: the isolated DNA of claim 1, wherein the anthranilate synthase contains a mutation that increases the activity of anthranilate synthase or reduces the sensitivity of the anthranilate synthase inhibition for tryptophan or an Analogue thereof.Claim 1: the isolated DNA of claim 9, wherein the mutation is within the positions of amino acids or 25 - 60 - 200 - 225 or 290 300 370 - 375 or when the amino acid sequence of the anthranilate synthase is aligned with a monomer of anthranilate synthase agr Obacterium tumefaciens with SEQ ID no: 2.Claim 17: the isolated DNA of claim 1, wherein the isolated DNA for gene also encodes a selectable Marker and reporter gene. Claim 19: the isolated DNA of claim 18, wherein the herbicide Resistance includes the resistance to glyphosate, glufosinate or Dalapon.Claim: 20 isolated DNA of claim 1, wherein the isolated DNA encodes for a Protein of Bacillus thuringiensis in addition that, when expressed in a plant is the plant resistance to insects.Claim 93: a method for altering the content of tryptophan in a plant and that involves: (b) introducing into a Plant a regenerable cells Transgene comprising an isolated DNA encoding an anthranilate synthase Alpha Domain monomer comprising an anthranilate s INTASA domain and a Beta of anthranilate synthase,Where the isolated DNA is operably linked to a promoter is functional in a Plant Cell to result in transformed plant cells; and (c) Regenerating a plant from the transformed plant cells.Where the plant cells express the anthranilate synthase encoded by the isolated DNA monomer amount effective to increase the content of tryptophan in the plant in relation to the content of tryptophan in a Plant transformed with the same background genu00e9ti Cos.Claim 149: a Food animal or human food, characterized in that it comprises at least a portion of a plant that contains an isolated DNA encoding a monomer comprising an anthranilate synthase domain of anthranilate synthase Alpha and beta Domain if anthranilate Ntasa,Where the plant cells can express the anthranilate synthase encoded by the isolated DNA monomer.