Vaksiner og antistoff produsert fra virus utledet fra en udodelig cellelinje fra grouper epinephelus coioides

摘要

The present invention provides for antibodies against nerous necrosis virus (NNV) and infectious pancreatic necrosis (IPNV) virus. The antibodies include polyclonal and monoclonal antibodies. NNV and IPNV are produced from an immortal cell line derived from Epinephelus coioides having an ATCC deposit number of PTA-859. The present invention also provides methods for detecting viral infections in fish using enzyme immunoassay (EIA). Additionally, the present invention provides for fish vaccines and methods of using the fish vaccines to immunize susceptible fish against viral infections. In particular, the present invention is directed to vaccines and methods of immunizing susceptible fish for nervous necrosis (VNN) disease which is caused by NNV, and infectious pancreatic necrosis (IPN) disease which is caused by IPNV. The vaccines are produced from the treatment of viruses that are mass-produced from an immortal cell line derived from Epinephelus coioides having an ATCC deposit number of PTA-859.