METHOD FOR DETECTING AND QUANTITATIVELY DETERMINING HEMOLYSIN-PRODUCING BACTERIA BY TOTALLY DETECTING AND QUANTITATIVELY DETERMINING THERMOSTABLE DIRECT HEMOLYSIN-RELATED (TDH-RELATED TOXIN GENE) OF FOOD POISONING BACTERIA

摘要

AbstractThe present invention simply, rapidly and accurately detects, quantitatively determines and types genes of protein groups [which refer to TDH (Thermostable direct hemolysin), TRH (TDH-related hemolysin) and other analogous hemolysin proteins, hereinafter referred to as TDH-related toxins] of thermostable hemolysin . groupsproduced by pathogenic bacteria of the genus VibroPresent Invention provides a method which enables detection and quantitative determination of actual TDH-related toxin-producing bacteria, which are important infood hygiene control.Using a primer pair which contains gene sequences encoding common amino acid sequences of TDH and TRH, TDH-related toxin genes are comprehensively detected and determined, thereby TDH-related toxin-producing bacteria themselves are detected and quantitative determined.