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Real-time pcr primers and probes for identification as ralstonia solanacearum race 3, biovar 2 in potato
Real-time pcr primers and probes for identification as ralstonia solanacearum race 3, biovar 2 in potato
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机译:实时pcr引物和探针,用于鉴定马铃薯马铃薯青枯菌第3种,biovar 2
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摘要
Ralstonia solanacearum, the causal agent of bacterial brown rot of potato, is often carried latently in seed potato tubers. Primers and a probe were designed for a real-time BIO-PCR assay technique for detecting potato tubers latently infected with R. Solanacearum. Using naturally infected potato tubers, as few as 20 cells/ml extract could be detected. Two of 14 naturally infected potato tubers with no disease symptoms were positive by the newly described real-time BIO-PCR (pre-enrichment on agar or in liquid medium) assay but not by direct real-time PCR.
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