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METHOD FOR TESTING SUBSTANCE WHICH IS POTENTIALLY ACTIVE IN THE FIELD OF LIPOLYSIS AND USE THEREOF FOR RESEARCH LABORATORIES
METHOD FOR TESTING SUBSTANCE WHICH IS POTENTIALLY ACTIVE IN THE FIELD OF LIPOLYSIS AND USE THEREOF FOR RESEARCH LABORATORIES
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机译:在脂解领域中可能具有活性的物质的测试方法及其在研究实验室中的使用
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摘要
PURPOSE: A method for testing a substance which is potentially active in the field of lipolysis and use thereof are provided, thereby safely and reliably testing the substance, and finding the substance with a method which has the lowest number of steps as possible, is not expensive, and can be used on an industrial scale, especially in research laboratories, preferably automatically. CONSTITUTION: The method of testing a substance which is potentially active in the field of lipolysis comprises the steps of: (a) preparing a substrate which contains at least one triacylglycerol; (b) placing this substrate in contact with a substance which is potentially active in the field of lipolysis, and with a lipoprotein lipase, in the presence of a cofactor of lipoprotein lipase, for a period of time sufficient for releasing at least in part one fatty acid of the triacylglycerol; and (c) determining the capacity of inhibition of the release of the fatty acid resulting from the activity of the lipoprotein lipase, under the action of the potentially active substance, and evaluating the results of the inhibition which are compared to the result obtained in the absence of the potentially active substance tested or which are compared with the result obtained in the presence of a known inhibitor acting as reference.
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