PRIMER SETS AND PROBES FOR DETECTION OF GMO, AND STANDARD PLASMID FOR QUALITATIVE AND QUANTITATIVE DETECTION OF GMO TO EFFECTIVELY CARRY OUT QUALITATIVE AND QUANTITATIVE DETECTION OF GMO IN AGRICULTURAL PRODUCTS AND FOODS

摘要

PURPOSE: Primer sets and probes for detection of GMO, and a standard plasmid for qualitative and quantitative detection of GMO are provided, thereby effectively carrying out qualitative and quantitative detection of GMO in agricultural products and foods. CONSTITUTION: The insect tolerant primer set for detection of GMO(genetically modified organism) plants comprises one or two primers selected from NLL-F primer of SEQ ID NO:1, NLL-R primer of SEQ ID NO:2, Cry3A-F primer of SEQ ID NO:6, Cry3A-R primer of SEQ ID NO:7 and Cry3A-F2 primer of SEQ ID NO:8. The Cyr3A-pb probe of SEQ ID NO:9 is used together with the insect tolerant primer set for detection of GMO plant. The UGP-pb probe of SEQ ID NO:14 is used together with the insect tolerant primer set for detection of GMO plant. The insect and virus tolerant primer set for detection of GMO plant such as a potato comprises one or two primers selected from NLYL-F primer of SEQ ID NO:3, NLYM-R primer of SEQ ID NO:4 and NLPS-R primer of SEQ ID NO:5. The primer set for detection of UDP-glucose phosphorylase gene of natural or GMO plants comprises one or two primers selected from UGP-F primer of SEQ ID NO:10, UGP-R primer of SEQ ID NO:11, UGP-F2 primer of SEQ ID NO:12 and UGP-R2 primer of SEQ ID NO:13. The standard plasmid pCryugp is used for qualitative and quantitative detection of GMO plants.