CATALYTIC DNA MOLECULE SHOWING SITE-SPECIFIC ENDONUCLEASE ACTIVITY (VARIANTS), COMPOSITION CLEAVING NUCLEIC ACID, METHOD FOR CLEAVING PHOSPHOESTER BOND IN SUBSTRATE, METHOD FOR SELECTION OF CATALYTIC DNA (VARIANTS), ENZYMATIC DNA MOLECULE (VARIANTS)

摘要

FIELD: biochemistry, enzymology, nucleic acids. SUBSTANCE: invention relates to enzymatic nucleic acids that are able to cleave nucleic acid sequences. Sequences of enzymatic nucleic acids are given in the description. Composition cleaving nucleic acid comprises catalytic DNA. Cleavage of phosphoester bond in substrate is carried out by mixing catalytic DNA molecule with substrate comprising such bond and the following incubation of mixture. Then molecules of catalytic DNA are separated from products of catalytic reaction. Selection of catalytic DNA molecules is carried out by mixing singlestranded molecules of catalytic DNA with nucleotide-containing substrate molecules followed by exposition and separation. Invention provides preparing molecules of catalytic DNA showing substratespecific endonuclease (enzymatic) activity. EFFECT: valuable biological and biochemical properties of nucleic acid. 66 cl, 11 dwg, 3 tbl, 5 ex