METHOD FOR SEPARATION OF HUMAN DNA-ASE (VARIANTS), PURIFIED DESAMIDATED HUMAN DNA-ASE, PURIFIED HUMAN DNA-ASE, PHARMACEUTICAL COMPOSITION FOR REDUCING PUS VISCOELASTICITY (VARIANTS), METHOD FOR TREATMENT OF PATIENT SUFFERING WITH PUS MASS (VARIANTS), METHOD FOR TREATMENT OF PATIENT SUFFERING WITH MUCOVISCEIDOSIS, BRONCHITIS, PNEUMONIA

摘要

FIELD: biotechnology, genetic engineering, medicine, biochemistry.;SUBSTANCE: invention relates to identification and characterization of two components of recombinant DNA-ase preparation. These components represent the purified desamidated and purified non-desamidated human DNAa-ase. Methods for separation of these DNA-ases involve using polydentate cation-exchange resin or using heparin immobilized on this resin, or non-hydrolyzable DNA analog immobilized on this resin. The use of non-desamidated DNA-ase as a component of the pharmaceutical composition is effective for administration to patients suffering with disturbances in pulmonary system.;EFFECT: improved separating method, improved treatment method, valuable medicinal properties of composition.;14 cl, 10 dwg, 3 tbl, 1 ex