Procedure for the configuration of parallel nucleic acid analysis procedures for sequential mass classification

摘要

Configuring nucleic acid for analysis of sequence classification, comprising selecting capture nucleic acid complementary to a range of target nucleic acids, is new. ??Configuring nucleic acid methods of analysis for sequence classification, comprises: ??(a) selecting capture nucleic acid complementary if possible to a range of target nucleic acids by the binding energy or binding probability; ??(b) selecting capture nucleic acid complementary if possible to a range of target nucleic acids in bulk serums containing a range of nucleic acids; ??(c) selecting capture nucleic acid complementary if possible to a range of target nucleic acids with consideration of at least one of the following criteria database quality, database sensitivity, database specificity, positive or negative correctness concerning the database, agreement, rate of false negatives or positives concerning the database, database diversity, redundancy level, tolerance level, distance difference, hybridizing initiation place, binding to the capture nucleic acid, relative length compared to the capture nucleic acid, accessibility of the target to the capture nucleic acid, relative length of the capture nucleic acid compared to the target and T-content; or ??(d) selecting capture nucleic acid complementary if possible to a range of target nucleic acids satisfying two or all three of (a) -(c)above whereby also known are one or a combination of the number of hits, fusion temperature, library size, secondary structure of the capture nucleic acid, capture/capture dimer, spacer or GC-binding. ??Independent claims are also included for: ??(i) a computer programme with code for carrying out the method of the invention; ??(ii) a computer programme product with computer code on a machine-readable container; ??(iii) a device which holds the computer programme; ??(iv) parallel nucleic acid for sequence classification which is configured using the method.